What Is ELISA Test?

ELISA is the abbreviation of the enzyme-linked immunosorbent assay. Sometimes, you would see another terminology EIA (enzyme immunoassay) instead of ELISA, they both represent the same meaning. It is firstly used in detecting autoimmune-related antibodies in patients with autoimmune disease.

With the help of bound enzymes, the reactions between antigens and antibodies can be shown in certain colors. For this reason, it is called "Enzyme-Linked Immunosorbent Assay" because the ELISA test is linked to "Immune" and "Enzyme". You can also get the testosterone elisa kit for rats from various online sources.

But as we can see, ELISA tests are not only used in the field to detect antigens/antibodies as they develop, and some ELISA tests use other methods to amplify signals instead of enzymes. associated with "immune" or "enzymatic" tests such as ELISA. Meanwhile, if we prefer to use this terminology, we need to expand the definition of ELISA.

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We can define an ELISA test as follows: “In an ELISA test, the ligand(s) is conjugated to the detected and measured color change receptor(s).” We use ligands and receptors to replace antigens and antibodies. and deletion of descriptions relating to enzymes, thereby broadening the scope of the definition.

The ELISA test is a typical "wet laboratory" test, although it uses a solid phase to detect the presence of a substance, the substance is usually in a liquid or wet sample. Usually, the solid phase is a 96-well solid plate, while some ELISA tests are performed with a 192-well or 384-well plate.

The ELISA test is widely used in medical diagnosis. And used to detect plant disease-causing pathogens in plant research. In addition, many industries, such as the food industry, use the ELISA test in the process of quality control and quality assurance.