CD27, a costimulatory molecule, is expressed constitutively on a majority of mature T cells and memory B cells. It also appears on a small percentage of NK cells. CD27 and its ligand CD70 play an important role in memory and effector capacity in T cells, clonal cell expansion and germinal centre formation, and in NK cell cell cytolytic activities.
Anti-tumor activity has been demonstrated to be potent in agonistic anti-CD27 antibodies. It has been demonstrated that fully human antibodies recognize CD27 and that the lead clone (1F5) can exert anti-tumor activity using the BCL1 B lymphoma line in CD27-transgenic (hCD27Tg) mice.
Antibodies that detect CD27 are useful in many scientific applications such as Western Blot, Flow Cytometry and Immunohistochemistry (Paraffin), Immunocytochemistry, ELISA, and Immunohistochemistry. These antibodies can target CD27 in human, mouse, rat, porcine and hamster samples.
CD27, a 50kDa member of the tumor necrosis factors (TNFs) receptor superfamily that also includes CD40 or CD30, is a 50kDa member. TNF superfamily members have been known to regulate cell proliferation and death. Contrary to other TNFR/TNF members, CD27 and its ligand CD70 are mainly expressed in lymphocytes.
The proper ligation and expression of antigen receptors is required for high levels of CD27 expression. Additional co-stimulatory or pro-inflammatory signals are required for CD70 expression. CD27 is expressed in a disulfide linked homodimer on mature and peripheral blood T cells, as well as subpopulation B cells.